Hotspot

rps16-trnQ-UUG

LSC · 5872-7264 · 1393 bp

Back to Yucca

Pi 0.00549
MarkerSeek score 64.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1393
variable_sites
22
pi
0.00549
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCATGTCCTTCAAGTCGCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1461-1486 bp 1.0 78.8
rps16-trnQ-UUG_p2 ATCATGTCCTTCAAGTCGCG GTCACTCGGAGGTTCGAATC 50.0 55.0 58.4 58.1 1469-1494 bp 1.0 78.7
rps16-trnQ-UUG_p3 ATCATGTCCTTCAAGTCGCG TAAACCTAAATGGGGCGTGG 50.0 50.0 58.4 57.9 1519-1544 bp 1.0 78.2
rps16-trnQ-UUG_p4 ATCATGTCCTTCAAGTCGCG CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.4 57.8 1465-1490 bp 1.0 77.7
rps16-trnQ-UUG_p5 GCGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1442-1467 bp 1.0 77.4