Hotspot

trnK-UUU-rps16

LSC · 4048-4741 · 694 bp

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Pi 0.00851
MarkerSeek score 60.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
694
variable_sites
14
pi
0.00851
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAA GGTGCTCAACCTACAGGAAC 45.0 55.0 58.1 58.2 808-812 bp 1.0 79.2
trnK-UUU-rps16_p2 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 783-787 bp 1.0 76.6
trnK-UUU-rps16_p3 TAGTCGCACTTAAAAGCCGA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 809-813 bp 1.0 74.4
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAA GTGCTCAACCTACAGGAACT 45.0 50.0 58.1 57.2 807-811 bp 1.0 74.0
trnK-UUU-rps16_p5 GCCGAATACTCTACCGTTGA GGTGCTCAACCTACAGGAAC 50.0 55.0 57.1 58.2 794-798 bp 1.0 73.4