Hotspot

ycf1

SSC · 125565-131156 · 5592 bp

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Pi 0.00948

MarkerSeek score 47.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 53
pi: 0.00948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAAGTAACCGTTTTGCGTC	ATGGACCCTATCGTGGAACA	50.0	50.0	58.0	58.1	695 bp	1.0	80.3
ycf1_p2	ACCGACCCAGTTTCGAAAAA	ATGGACCCTATCGTGGAACA	45.0	50.0	58.0	58.1	815 bp	1.0	80.1
ycf1_p3	CCAAGTAACCGTTTTGCGTC	TGAATGGACCCTATCGTGGA	50.0	50.0	58.0	57.8	698 bp	1.0	79.9
ycf1_p4	CCAAGTAACCGTTTTGCGTC	TGCCTGCTCAAAACGTATGA	50.0	45.0	58.0	57.8	726 bp	1.0	79.8
ycf1_p5	ACCGACCCAGTTTCGAAAAA	TGAATGGACCCTATCGTGGA	45.0	50.0	58.0	57.8	818 bp	1.0	79.8