Hotspot

rpl32-trnL_UAG

SSC · 114844-115704 · 861 bp

Back to Ypsilandra

Pi 0.0153

MarkerSeek score 67.40

Primer available yes

Label rpl32-trnL_UAG

Feature Metadata

label_name: rpl32-trnL_UAG
feature_type: igs
region: SSC
length_bp: 861
variable_sites: 13
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_UAG_p1	TTTTTGTGCGCCAAACAAGT	AGCGTGTCTACCGATTTCAC	40.0	50.0	57.9	58.0	911-919 bp	1.0	81.4
rpl32-trnL_UAG_p2	TTTTTGTGCGCCAAACAAGT	CAGCGTGTCTACCGATTTCA	40.0	50.0	57.9	58.0	912-920 bp	1.0	81.4
rpl32-trnL_UAG_p3	TTTTTGTGCGCCAAACAAGT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	57.9	57.9	921-929 bp	1.0	80.5
rpl32-trnL_UAG_p4	TTTTTGTGCGCCAAACAAGT	CCTAAGAGCAGCGTGTCTAC	40.0	55.0	57.9	57.8	920-928 bp	1.0	79.9
rpl32-trnL_UAG_p5	TTTTTGTGCGCCAAACAAGT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	57.9	57.8	919-927 bp	1.0	79.9