Hotspot

rpl2-rpl23

IRb · 86721-86738 · 18 bp

Back to Ypsilandra

Pi 0.0556

MarkerSeek score 81.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 18
variable_sites: 1
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TGCTCGGGGTAGAAGTTTTG	CCCGGGAAAGGGTAGAAGAA	50.0	55.0	57.8	58.7	173 bp	1.0	73.3
rpl2-rpl23_p2	GCTCGGGGTAGAAGTTTTGT	CCCGGGAAAGGGTAGAAGAA	50.0	55.0	57.8	58.7	172 bp	1.0	73.3
rpl2-rpl23_p3	GTGCTCGGGGTAGAAGTTTT	CCCGGGAAAGGGTAGAAGAA	50.0	55.0	57.8	58.7	174 bp	1.0	73.3
rpl2-rpl23_p4	TTGTCTATGGCTCCATTGCG	CCCGGGAAAGGGTAGAAGAA	50.0	55.0	58.3	58.7	195 bp	1.0	72.0
rpl2-rpl23_p5	TGCTCGGGGTAGAAGTTTTG	AGGGTAGAAGAATGGGACCT	50.0	50.0	57.8	57.0	165 bp	1.0	71.3