Hotspot

rps2-rpoC2

LSC · 15905-16063 · 159 bp

Back to Ypsilandra

Pi 0.019

MarkerSeek score 63.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 3
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCAGAAATGTAAGGGGCCAT	TATTTTGTTCTTGCGCCCCA	50.0	45.0	58.2	58.4	343-349 bp	1.0	76.1
rps2-rpoC2_p2	TAAGGGGCCATTCTAGGGTT	TATTTTGTTCTTGCGCCCCA	50.0	45.0	57.7	58.4	334-340 bp	1.0	75.5
rps2-rpoC2_p3	GTAAGGGGCCATTCTAGGGT	TATTTTGTTCTTGCGCCCCA	55.0	45.0	58.5	58.4	335-341 bp	1.0	74.1
rps2-rpoC2_p4	TGTAAGGGGCCATTCTAGGG	TATTTTGTTCTTGCGCCCCA	55.0	45.0	58.5	58.4	336-342 bp	1.0	74.1
rps2-rpoC2_p5	GCAGAAATGTAAGGGGCCAT	CCCCAAACAATTTCCACGAG	50.0	50.0	58.2	57.3	328-334 bp	1.0	73.4