Hotspot

atpA-atpF

LSC · 11582-11687 · 106 bp

Back to Ypsilandra

Pi 0.019

MarkerSeek score 63.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 106
variable_sites: 2
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	CTTGGGGCCATGGAAGAAAT	55.0	50.0	58.1	58.1	248-249 bp	1.0	79.2
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	TACAAGGGGCTCTAGGAACC	55.0	55.0	58.1	58.1	328-329 bp	1.0	79.0
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	CCTTACAAGGGGCTCTAGGA	55.0	55.0	58.1	57.8	331-332 bp	1.0	78.6
atpA-atpF_p4	TTGTTCAATACGCTCACGGA	CTTGGGGCCATGGAAGAAAT	45.0	50.0	57.6	58.1	192-193 bp	1.0	76.7
atpA-atpF_p5	TTGTTCAATACGCTCACGGA	TACAAGGGGCTCTAGGAACC	45.0	55.0	57.6	58.1	272-273 bp	1.0	76.3