Hotspot

ycf1

SSC · 123218-128233 · 5016 bp

Back to Youngia

Pi 0.016
MarkerSeek score 49.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5016
variable_sites
80
pi
0.016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGTTGTAGAGCGATTTTGCG ACACAATCAAAAATGAAATCGA 45.0 27.3 56.8 53.2 214-217 bp 1.0 43.3
ycf1_p2 AGTTGTAGAGCGATTTTGCG AGGATTGATAGAAGAAACACAATCA 45.0 32.0 56.8 56.5 230-233 bp 1.0 43.1
ycf1_p3 AAGTTGTAGAGCGATTTTGCG AGGATTGATAGAAGAAACACAATCA 42.9 32.0 57.5 56.5 231-234 bp 1.0 43.1
ycf1_p4 AGTTGTAGAGCGATTTTGCG GGATTGATAGAAGAAACACAATCA 45.0 33.3 56.8 55.2 229-232 bp 1.0 43.1
ycf1_p5 AGTTGTAGAGCGATTTTGCG AGGATTGATAGAAGAAACACA 45.0 33.3 56.8 52.2 230-233 bp 1.0 43.1