Hotspot

rpl32-trnL-UAG

SSC · 112828-113625 · 798 bp

Back to Youngia

Pi 0.036
MarkerSeek score 73.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
798
variable_sites
28
pi
0.036
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 913-1042 bp 1.0 80.7
rpl32-trnL-UAG_p2 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 914-1043 bp 1.0 80.7
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.6 57.9 923-1052 bp 1.0 79.8
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.6 57.8 922-1051 bp 1.0 79.2
rpl32-trnL-UAG_p5 CGGCGTTAAAAGCTTTGTCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.6 57.8 921-1050 bp 1.0 79.2