Hotspot

petA-psbJ

LSC · 63194-63960 · 767 bp

Back to Youngia

Pi 0.0235

MarkerSeek score 72.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 18
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	905-906 bp	1.0	78.4
petA-psbJ_p2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	943-944 bp	1.0	76.7
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	942-943 bp	1.0	76.6
petA-psbJ_p4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	911-912 bp	1.0	75.6
petA-psbJ_p5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	910-911 bp	1.0	75.6