Hotspot

psbI-trnS-GCU

LSC · 8415-8553 · 139 bp

Back to Youngia

Pi 0.0288

MarkerSeek score 69.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 4
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TCGAATCCCTCTCTTTCCGT	50.0	50.0	57.1	58.2	183-189 bp	1.0	73.0
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	256-262 bp	1.0	72.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	204-210 bp	1.0	70.9
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	TCGAATCCCTCTCTTTCCGT	47.6	50.0	57.8	58.2	183-189 bp	1.0	70.7
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	256-262 bp	1.0	70.5