Hotspot

trnK-UUU-rps16

LSC · 4384-5203 · 820 bp

Back to Youngia

Pi 0.0224

MarkerSeek score 62.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 18
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGAACTTTGCCCTAATCAAACG	55.0	45.5	57.9	58.2	935-948 bp	1.0	64.9
trnK-UUU-rps16_p2	CGATGGTATGGACGAATCCC	GGAACTTTGCCCTAATCAAACG	55.0	45.5	57.9	58.2	934-947 bp	1.0	64.9
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	GGAACTTTGCCCTAATCAAACG	50.0	45.5	58.2	58.2	875-888 bp	1.0	64.4
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GGAACTTTGCCCTAATCAAACG	50.0	45.5	58.2	58.2	874-887 bp	1.0	64.4
trnK-UUU-rps16_p5	TCAGTCGTGGTCTTCCAAAC	GGAACTTTGCCCTAATCAAACG	50.0	45.5	57.8	58.2	959-972 bp	1.0	63.6