Hotspot

trnH-GUG-psbA

LSC · 77-471 · 395 bp

Back to Youngia

Pi 0.0287
MarkerSeek score 69.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
395
variable_sites
10
pi
0.0287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 472-514 bp 1.0 81.9
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 486-528 bp 1.0 81.8
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 485-527 bp 1.0 81.8
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 487-529 bp 1.0 81.8
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 473-515 bp 1.0 81.4