Hotspot

trnL-CAA-rps16

Genome · 40691-44933 · 4243 bp

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Pi 0.0203
MarkerSeek score 45.60
Primer available yes
Label trnL-CAA-rps16

Feature Metadata

label_name
trnL-CAA-rps16
feature_type
igs
region
Genome
length_bp
4243
variable_sites
95
pi
0.0203
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.50
markerseek_score
45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-rps16_p1 ATCGGCAATGGGTCTGAAAG GGTCCGAGGCTATTGACAAA 50.0 50.0 58.2 57.6 1054 bp 0.25 44.9
trnL-CAA-rps16_p2 ATCGGCAATGGGTCTGAAAG ATTGGTCCGAGGCTATTGAC 50.0 50.0 58.2 57.4 1057 bp 0.25 43.1
trnL-CAA-rps16_p3 CATCGGCAATGGGTCTGAAA GGTCCGAGGCTATTGACAAA 50.0 50.0 58.5 57.6 1055 bp 0.25 42.5
trnL-CAA-rps16_p4 ATCGGCAATGGGTCTGAAAG TGGTCCGAGGCTATTGACAA 50.0 50.0 58.2 58.7 1055 bp 0.25 42.2
trnL-CAA-rps16_p5 ATCGGCAATGGGTCTGAAAG TTGGTCCGAGGCTATTGACA 50.0 50.0 58.2 58.7 1056 bp 0.25 42.2