Hotspot

rrn4.5-rrn5

Genome · 32248-32387 · 140 bp

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Pi 0.127
MarkerSeek score 79.60
Primer available yes
Label rrn4.5-rrn5

Feature Metadata

label_name
rrn4.5-rrn5
feature_type
igs
region
Genome
length_bp
140
variable_sites
31
pi
0.127
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5-rrn5_p1 TGAGGCATCCTAACAGACCA GCCTAGGACACCAGAATA 50.0 50.0 58.0 52.5 191-289 bp 1.0 51.0
rrn4.5-rrn5_p2 CAGCTGAGGCATCCTAACAG GCCTAGGACACCAGAATA 55.0 50.0 58.0 52.5 195-293 bp 1.0 51.0
rrn4.5-rrn5_p3 GCTGAGGCATCCTAACAGAC GCCTAGGACACCAGAATA 55.0 50.0 58.1 52.5 193-291 bp 1.0 51.0
rrn4.5-rrn5_p4 AGCTGAGGCATCCTAACAGA GCCTAGGACACCAGAATA 50.0 50.0 58.1 52.5 194-292 bp 1.0 51.0
rrn4.5-rrn5_p5 CTGAGGCATCCTAACAGACC GCCTAGGACACCAGAATA 55.0 50.0 57.4 52.5 192-290 bp 1.0 51.0