Hotspot

rpl16-rps3

Genome · 14194-15366 · 1173 bp

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Pi 0.0485
MarkerSeek score 82.00
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
Genome
length_bp
1173
variable_sites
95
pi
0.0485
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CCTCGGCAAGATATTCCCTT TCAAAATTTGGATATTTGTAAACCCA 50.0 26.9 57.1 56.3 1227-1319 bp 1.0 50.0
rpl16-rps3_p2 GCCTCGGCAAGATATTCCC TCAAAATTTGGATATTTGTAAACCCA 57.9 26.9 58.1 56.3 1228-1320 bp 1.0 50.0
rpl16-rps3_p3 CCTCGGCAAGATATTCCCTTC TCAAAATTTGGATATTTGTAAACCCA 52.4 26.9 58.2 56.3 1227-1319 bp 1.0 50.0
rpl16-rps3_p4 CCTCGGCAAGATATTCCCTT CAAAATTTGGATATTTGTAAACCCA 50.0 28.0 57.1 55.0 1226-1318 bp 1.0 50.0
rpl16-rps3_p5 GCCTCGGCAAGATATTCCC CAAAATTTGGATATTTGTAAACCCA 57.9 28.0 58.1 55.0 1227-1319 bp 1.0 50.0