Hotspot

trnfM-CAU-rps14

Genome · 3243-3393 · 151 bp

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Pi 0.116
MarkerSeek score 75.10
Primer available yes
Label trnfM-CAU-rps14

Feature Metadata

label_name
trnfM-CAU-rps14
feature_type
igs
region
Genome
length_bp
151
variable_sites
29
pi
0.116
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.87
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rps14_p1 CCCGTGGTCTCAAGGTTATG CACGTAATAGTGCGCGGATA 55.0 50.0 58.0 58.0 342-377 bp 1.0 91.5
trnfM-CAU-rps14_p2 CCCGTGGTCTCAAGGTTATG ACGTAATAGTGCGCGGATAC 55.0 50.0 58.0 58.0 341-376 bp 1.0 91.5
trnfM-CAU-rps14_p3 AAGGTTATGAGCCTTGCGAG CACGTAATAGTGCGCGGATA 50.0 50.0 58.0 58.0 331-366 bp 1.0 91.4
trnfM-CAU-rps14_p4 AAGGTTATGAGCCTTGCGAG ACGTAATAGTGCGCGGATAC 50.0 50.0 58.0 58.0 330-365 bp 1.0 91.4
trnfM-CAU-rps14_p5 GTTGAATCCCCGTTACCTCC CACGTAATAGTGCGCGGATA 55.0 50.0 58.0 58.0 502-546 bp 1.0 90.1