Hotspot

trnE-UUC-trnfM-CAU

Genome · 3089-3168 · 80 bp

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Pi 0.0633
MarkerSeek score 78.50
Primer available yes
Label trnE-UUC-trnfM-CAU

Feature Metadata

label_name
trnE-UUC-trnfM-CAU
feature_type
igs
region
Genome
length_bp
80
variable_sites
9
pi
0.0633
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnfM-CAU_p1 TGTCCTGAACCACTAGACGA CTTGAGACCACGGGTTCAAA 50.0 50.0 57.7 58.0 140-151 bp 1.0 83.5
trnE-UUC-trnfM-CAU_p2 TGTCCTGAACCACTAGACGA CATAACCTTGAGACCACGGG 50.0 55.0 57.7 58.0 146-157 bp 1.0 83.4
trnE-UUC-trnfM-CAU_p3 TGTCCTGAACCACTAGACGA GTTCAAATCCTGTCTCCGCA 50.0 50.0 57.7 58.2 127-138 bp 1.0 82.9
trnE-UUC-trnfM-CAU_p4 TGTCCTGAACCACTAGACGA CACGGGTTCAAATCCTGTCT 50.0 50.0 57.7 57.8 132-143 bp 1.0 82.7
trnE-UUC-trnfM-CAU_p5 TGTCCTGAACCACTAGACGA TGAGACCACGGGTTCAAATC 50.0 50.0 57.7 57.8 138-149 bp 1.0 82.5