Pi
0.000218
MarkerSeek score
52.70
Primer available
yes
Label
ycf2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf2_p1 | AGGAGGAATCAATGAAAGGACA | GTTGAACCCTGTGAATTGCG | 40.9 | 50.0 | 57.1 | 57.9 | 220 bp | 1.0 | 56.5 |
| ycf2_p2 | AGGAGGAATCAATGAAAGGACA | TGCTTGTTGAACCCTGTGAA | 40.9 | 45.0 | 57.1 | 57.9 | 225 bp | 1.0 | 55.8 |
| ycf2_p3 | AGGAGGAATCAATGAAAGGACA | TTGCTTGTTGAACCCTGTGA | 40.9 | 45.0 | 57.1 | 57.9 | 226 bp | 1.0 | 55.8 |
| ycf2_p4 | AGGAGGAATCAATGAAAGGACA | CCTGTGAATTGCGCAAAAGT | 40.9 | 45.0 | 57.1 | 57.9 | 213 bp | 1.0 | 55.8 |
| ycf2_p5 | AGGAGGAATCAATGAAAGGACA | TAGAAGGGCCGCTACTACAA | 40.9 | 50.0 | 57.1 | 57.9 | 278 bp | 1.0 | 55.6 |
| ycf2_p1 | CCTGGAGAGAGAGTTCACCA | GTTCCTGGATAACAAGCCGA | 55.0 | 50.0 | 58.1 | 57.6 | 301 bp | 1.0 | 76.5 |
| ycf2_p2 | CCTGGAGAGAGAGTTCACCA | AGTTCCTGGATAACAAGCCG | 55.0 | 50.0 | 58.1 | 57.6 | 302 bp | 1.0 | 76.5 |
| ycf2_p3 | ACCAATAGACCGAGGGCTAA | GTTCCTGGATAACAAGCCGA | 50.0 | 50.0 | 57.8 | 57.6 | 285 bp | 1.0 | 75.7 |
| ycf2_p4 | ACCAATAGACCGAGGGCTAA | AGTTCCTGGATAACAAGCCG | 50.0 | 50.0 | 57.8 | 57.6 | 286 bp | 1.0 | 75.7 |
| ycf2_p5 | TCCCTGGAGAGAGAGTTCAC | GTTCCTGGATAACAAGCCGA | 55.0 | 50.0 | 57.8 | 57.6 | 303 bp | 1.0 | 75.3 |