Hotspot

ycf1

SSC · 124540-129921 · 5382 bp

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Pi 0.00198

MarkerSeek score 44.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5382
variable_sites: 20
pi: 0.00198
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGTCTAGGCTCATCTCCCC	TTACCTCAGATTGCATGGGC	55.0	50.0	58.0	57.9	1085 bp	1.0	80.0
ycf1_p2	CCGGCCGATTGTTTTTCTTC	TTACCTCAGATTGCATGGGC	50.0	50.0	58.0	57.9	767 bp	1.0	80.0
ycf1_p3	TCCATCGCAATTGAAAACGC	TTACCTCAGATTGCATGGGC	45.0	50.0	58.0	57.9	964 bp	1.0	79.9
ycf1_p4	ACAGACAAACGGACAGGTTC	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	1166 bp	1.0	79.8
ycf1_p5	CAGACAAACGGACAGGTTCA	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	1165 bp	1.0	79.7