Hotspot

rps16

LSC · 5040-6194 · 1155 bp

Back to Yinshania

Pi 0.00231

MarkerSeek score 51.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1155
variable_sites: 5
pi: 0.00231
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAGCGAAAGAGGAGGAAAGA	ACGCGTAAATTCACTATTCCA	45.0	38.1	56.5	55.7	1361-1362 bp	1.0	41.8
rps16_p2	AGCGAAAGAGGAGGAAAGAA	ACGCGTAAATTCACTATTCCA	45.0	38.1	56.5	55.7	1360-1361 bp	1.0	41.8
rps16_p3	AAGCGAAAGAGGAGGAAAGAA	ACGCGTAAATTCACTATTCCA	42.9	38.1	57.2	55.7	1361-1362 bp	1.0	40.3
rps16_p4	AGCGAAAGAGGAGGAAAGAAA	ACGCGTAAATTCACTATTCCA	42.9	38.1	57.2	55.7	1360-1361 bp	1.0	40.3
rps16_p5	AGCGAAAGAGGAGGAAAGAA	ACGCGTAAATTCACTATTCCAT	45.0	36.4	56.5	56.2	1360-1361 bp	1.0	40.3