Hotspot

rps16-trnQ-CAA

LSC · 6195-6696 · 502 bp

Back to Yinshania

Pi 0.00754

MarkerSeek score 51.50

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 7
pi: 0.00754
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	541-575 bp	1.0	83.3
rps16-trnQ-CAA_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	551-585 bp	1.0	82.5
rps16-trnQ-CAA_p3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	567-601 bp	1.0	81.0
rps16-trnQ-CAA_p4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	568-602 bp	1.0	81.0
rps16-trnQ-CAA_p5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	569-603 bp	1.0	79.3