Hotspot

rps15-ycf1

SSC · 123002-123386 · 385 bp

Back to Yamazakia

Pi 0.0443
MarkerSeek score 69.50
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
385
variable_sites
17
pi
0.0443
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CAGACCCTCTGTTTTCTTCGT GGAAGTCGTTTCAGCATGTT 47.6 45.0 57.9 56.6 509-513 bp 1.0 63.1
rps15-ycf1_p2 AGACCCTCTGTTTTCTTCGT GGAAGTCGTTTCAGCATGTT 45.0 45.0 56.4 56.6 508-512 bp 1.0 59.2
rps15-ycf1_p3 CAGACCCTCTGTTTTCTTCGT AGTCGTTTCAGCATGTTAAGGA 47.6 40.9 57.9 58.0 506-510 bp 1.0 57.9
rps15-ycf1_p4 AGACCCTCTGTTTTCTTCGTT GGAAGTCGTTTCAGCATGTT 42.9 45.0 57.2 56.6 508-512 bp 1.0 57.3
rps15-ycf1_p5 CAGACCCTCTGTTTTCTTCG GGAAGTCGTTTCAGCATGTT 50.0 45.0 56.1 56.6 509-513 bp 1.0 56.8