Hotspot

rps11-rpl36

LSC · 80768-80878 · 111 bp

Back to Yamazakia

Pi 0.0891

MarkerSeek score 74.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 9
pi: 0.0891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACTTCCACTGTAGTGTCCGA	AGGTGGGATGGGGATTGTAA	50.0	50.0	58.0	58.0	1310-1315 bp	1.0	82.0
rps11-rpl36_p2	TTCCACTGTAGTGTCCGAGT	AGGTGGGATGGGGATTGTAA	50.0	50.0	58.0	58.0	1308-1313 bp	1.0	82.0
rps11-rpl36_p3	ACTTCCACTGTAGTGTCCGA	GCTTCCAAATGGCATGTTCC	50.0	50.0	58.0	58.0	1039-1044 bp	1.0	82.0
rps11-rpl36_p4	TTCCACTGTAGTGTCCGAGT	GCTTCCAAATGGCATGTTCC	50.0	50.0	58.0	58.0	1037-1042 bp	1.0	82.0
rps11-rpl36_p5	TTCCACTGTAGTGTCCGAGT	TCAAGGGAGCTTTACCTGGA	50.0	50.0	58.0	58.0	1101-1106 bp	1.0	81.7