Hotspot

rps18-rpl20

LSC · 69753-69983 · 231 bp

Back to Yamazakia

Pi 0.0476

MarkerSeek score 70.40

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 11
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TGAAAGAACCGAGTCAACCG	TGGAAAGAATACACCGGAAA	50.0	40.0	58.1	54.6	313 bp	0.5	31.5
rps18-rpl20_p2	TGAAAGAACCGAGTCAACCG	TTGGAAAGAATACACCGGAA	50.0	40.0	58.1	54.6	314 bp	0.5	31.5
rps18-rpl20_p3	TGAAAGAACCGAGTCAACCG	TGGAAAGAATACACCGGAAAA	50.0	38.1	58.1	55.4	313 bp	0.5	30.2
rps18-rpl20_p4	TGAAAGAACCGAGTCAACCG	TTGGAAAGAATACACCGGAAA	50.0	38.1	58.1	55.4	314 bp	0.5	30.2
rps18-rpl20_p5	TGAAAGAACCGAGTCAACCG	TGGAAAGAATACACCGGAAAAA	50.0	36.4	58.1	56.2	313 bp	0.5	28.4