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psaJ-rpl33

LSC · 68591-69086 · 496 bp

Back to Yamazakia

Pi 0.0467

MarkerSeek score 69.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 23
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	CGAACAAACGACCAATCTGC	45.0	50.0	58.1	58.0	1261 bp	0.5	59.0
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	GAACAAACGACCAATCTGCG	45.0	50.0	58.1	58.0	1260 bp	0.5	59.0
psaJ-rpl33_p3	ACCCGTGACATTTTGTACCC	CGAACAAACGACCAATCTGC	50.0	50.0	58.1	58.0	1843 bp	0.5	58.8
psaJ-rpl33_p4	ACCCGTGACATTTTGTACCC	GAACAAACGACCAATCTGCG	50.0	50.0	58.1	58.0	1842 bp	0.5	58.8
psaJ-rpl33_p5	CCCGTGACATTTTGTACCCA	GAACAAACGACCAATCTGCG	50.0	50.0	58.1	58.0	1841 bp	0.5	58.8