Hotspot

trnP-UGG-psaJ

LSC · 68032-68455 · 424 bp

Back to Yamazakia

Pi 0.0259

MarkerSeek score 61.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 11
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CTAAAGCCCCGAACCATAGC	50.0	55.0	58.1	58.4	546-567 bp	1.0	77.5
trnP-UGG-psaJ_p2	ACCCGTGACATTTTGTACCC	GCTAAAGCCCCGAACCATAG	50.0	55.0	58.1	58.4	547-568 bp	1.0	77.5
trnP-UGG-psaJ_p3	CCCGTGACATTTTGTACCCA	CTAAAGCCCCGAACCATAGC	50.0	55.0	58.1	58.4	545-566 bp	1.0	77.5
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	GCTAAAGCCCCGAACCATAG	50.0	55.0	58.1	58.4	546-567 bp	1.0	77.5
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	CTAAAGCCCCGAACCATAGC	50.0	55.0	58.1	58.4	557-578 bp	1.0	77.4