Hotspot

trnW-CCA-trnP-UGG

LSC · 67747-67957 · 211 bp

Back to Yamazakia

Pi 0.109
MarkerSeek score 83.90
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
211
variable_sites
23
pi
0.109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 CGCTCTGTAGGACTTGAACC GTCACGGGTTCAAATCCTGT 55.0 50.0 58.0 58.1 311-353 bp 1.0 84.7
trnW-CCA-trnP-UGG_p2 CGCTCTGTAGGACTTGAACC CACGGGTTCAAATCCTGTCA 55.0 50.0 58.0 58.1 309-351 bp 1.0 84.7
trnW-CCA-trnP-UGG_p3 CGCTCTGTAGGACTTGAACC TGTCACGGGTTCAAATCCTG 55.0 50.0 58.0 58.1 312-354 bp 1.0 84.6
trnW-CCA-trnP-UGG_p4 CGCTCTGTAGGACTTGAACC GGGTACAAAATGTCACGGGT 55.0 50.0 58.0 58.1 322-364 bp 1.0 84.5
trnW-CCA-trnP-UGG_p5 CGCTCTGTAGGACTTGAACC TGGGTACAAAATGTCACGGG 55.0 50.0 58.0 58.1 323-365 bp 1.0 84.5