Hotspot

trnT-UGU-trnL-UAA

LSC · 47430-48147 · 718 bp

Back to Yamazakia

Pi 0.0376

MarkerSeek score 77.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 26
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	754-779 bp	1.0	82.5
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	786-811 bp	1.0	81.4
trnT-UGU-trnL-UAA_p3	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	805-830 bp	1.0	81.3
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	748-773 bp	1.0	81.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	786-811 bp	1.0	80.2