Hotspot

trnY-GUA-trnE-UUC

LSC · 31692-31750 · 59 bp

Back to Yamazakia

Pi 0.0508

MarkerSeek score 70.10

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 3
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	55.0	55.0	58.4	58.3	147 bp	1.0	76.7
trnY-GUA-trnE-UUC_p2	ACGAATTTACAGTCCGTCCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.6	58.3	148 bp	1.0	76.5
trnY-GUA-trnE-UUC_p3	GCCAACGAATTTACAGTCCG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.5	58.3	152 bp	1.0	75.3
trnY-GUA-trnE-UUC_p4	CGAATTTACAGTCCGTCCCC	GATAGTATGAGGGCGGTTGG	55.0	55.0	58.4	57.5	203 bp	1.0	74.7
trnY-GUA-trnE-UUC_p5	ACGAATTTACAGTCCGTCCC	GATAGTATGAGGGCGGTTGG	50.0	55.0	57.6	57.5	204 bp	1.0	74.5