Hotspot

trnD-GUC-trnY-GUA

LSC · 31507-31607 · 101 bp

Back to Yamazakia

Pi 0.0693
MarkerSeek score 71.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
101
variable_sites
7
pi
0.0693
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA CTGGTTCAAATCCAGCTCGG 50.0 55.0 58.5 58.9 155 bp 1.0 72.5
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA TCTACGCTGGTTCAAATCCA 50.0 45.0 58.5 56.9 161 bp 1.0 70.5
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA TGGTTCAAATCCAGCTCGG 50.0 52.6 58.5 57.8 154 bp 1.0 69.8
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GTCTACGCTGGTTCAAATCCA 50.0 47.6 58.5 58.3 162 bp 1.0 69.2
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA TGTCTACGCTGGTTCAAATCC 50.0 47.6 58.5 58.3 163 bp 1.0 69.2