Hotspot

psbI-trnS-GCU

LSC · 8585-8708 · 124 bp

Back to Yamazakia

Pi 0.0541

MarkerSeek score 70.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 6
pi: 0.0541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	224-237 bp	1.0	85.9
psbI-trnS-GCU_p2	CTTTTGTTTGGCAAGCTGCT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.1	58.0	726-739 bp	1.0	82.5
psbI-trnS-GCU_p3	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	721-734 bp	1.0	82.5
psbI-trnS-GCU_p4	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	722-735 bp	1.0	82.5
psbI-trnS-GCU_p5	CTTTTGTTTGGCAAGCTGCT	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.1	58.0	727-740 bp	1.0	82.4