Hotspot

trnK-UUU-rps16

LSC · 4180-5126 · 947 bp

Back to Yamazakia

Pi 0.0407
MarkerSeek score 80.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
947
variable_sites
37
pi
0.0407
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 1050-1066 bp 1.0 80.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 1049-1065 bp 1.0 80.6
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.3 57.5 1064-1080 bp 1.0 79.8
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.3 57.5 1059-1075 bp 1.0 79.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAAGGGGCTCAACCTACAG 50.0 50.0 58.2 57.4 1045-1061 bp 1.0 79.7