Hotspot

rps15-trnN-GUU

SSC · 121298-122688 · 1391 bp

Back to Xyris

Pi 0.183
MarkerSeek score 83.60
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
1391
variable_sites
230
pi
0.183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 TGTGGAATAAATGAAATAAACGAGT CCACTGAGCTACTGAGGA 28.0 55.6 55.6 54.8 1444-2187 bp 1.0 52.0
rps15-trnN-GUU_p2 TGAAATAAACGAGTTTTTGATCA CCACTGAGCTACTGAGGA 26.1 55.6 53.2 54.8 1433-2176 bp 1.0 52.0
rps15-trnN-GUU_p3 TGTGGAATAAATGAAATAAACGAGTT CCACTGAGCTACTGAGGA 26.9 55.6 56.2 54.8 1444-2187 bp 1.0 52.0
rps15-trnN-GUU_p4 TTGTGGAATAAATGAAATAAACGAGT CCACTGAGCTACTGAGGA 26.9 55.6 56.2 54.8 1445-2188 bp 1.0 52.0
rps15-trnN-GUU_p5 CTTGTGGAATAAATGAAATAAACGAGT CCACTGAGCTACTGAGGA 29.6 55.6 57.2 54.8 1446-2189 bp 1.0 52.0