Hotspot

trnE-UUC-trnT-GGU

LSC · 59189-60089 · 901 bp

Back to Xyris

Pi 0.116
MarkerSeek score 88.60
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
901
variable_sites
93
pi
0.116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1005-1098 bp 1.0 89.7
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 983-1076 bp 1.0 88.9
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 1002-1095 bp 1.0 88.5
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 50.0 55.0 57.7 58.2 980-1073 bp 1.0 87.8
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA TTGAACCGATGACTTACGCC 50.0 50.0 57.8 58.3 1007-1100 bp 1.0 87.4