Hotspot

trnG-GCC-trnfM-CAU

LSC · 58100-58412 · 313 bp

Back to Xyris

Pi 0.144
MarkerSeek score 84.10
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
313
variable_sites
42
pi
0.144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GCAGTTTGGTAGCTTGCAAG 50.0 50.0 58.1 57.9 402-408 bp 1.0 91.1
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GCAGTTTGGTAGCTTGCAAG 50.0 50.0 58.1 57.9 403-409 bp 1.0 91.1
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC TTTCTTTCCTTTAGCGCGGA 50.0 45.0 58.1 57.8 427-433 bp 1.0 89.9
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT TTTCTTTCCTTTAGCGCGGA 50.0 45.0 58.1 57.8 428-434 bp 1.0 89.8
trnG-GCC-trnfM-CAU_p5 AGGAACGAAGAAAATGCGGA GCAGTTTGGTAGCTTGCAAG 45.0 50.0 57.8 57.9 456-462 bp 1.0 89.1