Hotspot

psbM-trnD-GUC

LSC · 30578-31513 · 936 bp

Back to Xyris

Pi 0.109
MarkerSeek score 83.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
936
variable_sites
98
pi
0.109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCGTTTTGGCTGACTGTTTT ATGCATGTTTGTTACCGGGT 40.0 45.0 56.4 58.1 1286-1462 bp 1.0 75.6
psbM-trnD-GUC_p2 TCGTTTTGGCTGACTGTTTT GGGCTATCCGGACGTATACT 40.0 55.0 56.4 58.2 1320-1496 bp 1.0 74.7
psbM-trnD-GUC_p3 TCGTTTTGGCTGACTGTTTT AGATCACAGAAAGGCCCTCT 40.0 50.0 56.4 58.0 1193 bp 0.5 46.9
psbM-trnD-GUC_p4 TCTGGAACTGGAAACTGGAA AGATCACAGAAAGGCCCTCT 45.0 50.0 56.3 58.0 1400 bp 0.5 46.0
psbM-trnD-GUC_p5 TTCTGGAACTGGAAACTGGA AGATCACAGAAAGGCCCTCT 45.0 50.0 56.3 58.0 1401 bp 0.5 46.0