Hotspot

petN-trnC-GCA

LSC · 15055-15522 · 468 bp

Back to Xyris

Pi 0.0995
MarkerSeek score 85.80
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
468
variable_sites
44
pi
0.0995
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 ACTACCATTAGAGCAGCCCA CTGCAAATCCTTTACCCCCA 50.0 50.0 58.1 57.8 550-556 bp 1.0 87.3
petN-trnC-GCA_p2 ACTACCATTAGAGCAGCCCA TGCAAATCCTTTACCCCCAG 50.0 50.0 58.1 57.8 549-555 bp 1.0 87.3
petN-trnC-GCA_p3 ACTACCATTAGAGCAGCCCA ACTGCAAATCCTTTACCCCC 50.0 50.0 58.1 57.8 551-557 bp 1.0 87.3
petN-trnC-GCA_p4 ACTACCATTAGAGCAGCCCA GCAAATCCTTTACCCCCAGT 50.0 50.0 58.1 57.8 548-554 bp 1.0 87.3
petN-trnC-GCA_p5 CAGCCCAAGCAAGACTTACT TGCAAATCCTTTACCCCCAG 50.0 50.0 57.8 57.8 536-542 bp 1.0 87.0