Hotspot

ycf1

SSC · 110351-112171 · 1821 bp

Back to Xylosma

Pi 0.0127

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 1821
variable_sites: 23
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	CAATGGAGTCGGCTTTACGA	55.0	50.0	58.0	58.0	2326-2348 bp	1.0	81.6
ycf1_p2	CCCGGAACTTCTTCGTAGTG	CAATGGAGTCGGCTTTACGA	55.0	50.0	58.0	58.0	2231-2253 bp	1.0	81.6
ycf1_p3	AACCCACGACCAATATGAGC	CAATGGAGTCGGCTTTACGA	50.0	50.0	58.0	58.0	2271-2293 bp	1.0	81.4
ycf1_p4	GAGCTACTGAGGAACAACGG	GGAGTCGGCTTTACGAGTTT	55.0	50.0	58.0	57.9	2322-2344 bp	1.0	81.1
ycf1_p5	CCCGGAACTTCTTCGTAGTG	GGAGTCGGCTTTACGAGTTT	55.0	50.0	58.0	57.9	2227-2249 bp	1.0	81.1