Hotspot

psbA

LSC · 328-1389 · 1062 bp

Back to Xylosma

Pi 0.00565

MarkerSeek score 50.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 6
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGCTCATACACACTAAGACCA	AGAATTCGTGTGCTTGGGAG	42.9	50.0	56.1	58.2	1140-1175 bp	1.0	55.7
psbA_p2	AGCTCATACACACTAAGACCA	AGAGAATTCGTGTGCTTGGG	42.9	50.0	56.1	58.2	1142-1177 bp	1.0	55.7
psbA_p3	AGCTCATACACACTAAGACCA	GAGAATTCGTGTGCTTGGGA	42.9	50.0	56.1	58.2	1141-1176 bp	1.0	55.7
psbA_p4	AGCTCATACACACTAAGACCAA	AGAATTCGTGTGCTTGGGAG	40.9	50.0	56.8	58.2	1140-1175 bp	1.0	53.7
psbA_p5	AGCTCATACACACTAAGACCAA	AGAGAATTCGTGTGCTTGGG	40.9	50.0	56.8	58.2	1142-1177 bp	1.0	53.7