Hotspot

trnN-GUU-trnR-ACG

SSC · 130980-131644 · 665 bp

Back to Xylosma

Pi 0.0271

MarkerSeek score 74.30

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 665
variable_sites: 18
pi: 0.0271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	842-854 bp	1.0	81.1
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	806-818 bp	1.0	80.0
trnN-GUU-trnR-ACG_p3	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	806-818 bp	1.0	80.0
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	793-805 bp	1.0	79.9
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	AACAGATCCAGTGGAGACGA	55.0	50.0	57.8	58.1	794-806 bp	1.0	79.6