Hotspot

ndhG-ndhI

SSC · 119784-120182 · 399 bp

Back to Xylosma

Pi 0.0125

MarkerSeek score 47.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 5
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CGTTCCCAACAGTCCAAAGA	TGGCAACTGCGTTGAGTATT	50.0	45.0	58.0	58.1	902-914 bp	1.0	81.2
ndhG-ndhI_p2	CGTTCCCAACAGTCCAAAGA	TGGTCAACAAACCATACGGG	50.0	50.0	58.0	58.1	1190-1202 bp	1.0	80.8
ndhG-ndhI_p3	GCAATCGCACCTATCAAAGC	TGGCAACTGCGTTGAGTATT	50.0	45.0	57.9	58.1	1125-1137 bp	1.0	80.3
ndhG-ndhI_p4	GCAATCGCACCTATCAAAGC	TGGTCAACAAACCATACGGG	50.0	50.0	57.9	58.1	1413-1425 bp	1.0	80.1
ndhG-ndhI_p5	TGGCAATCGCACCTATCAAA	TGGTCAACAAACCATACGGG	45.0	50.0	57.9	58.1	1415-1427 bp	1.0	80.0