Hotspot

ndhD-psaC

SSC · 118114-118222 · 109 bp

Back to Xylosma

Pi 0.0917

MarkerSeek score 86.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 109
variable_sites: 10
pi: 0.0917
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCGGATATTGGTAA	AAACAACTCGAAGCATGGGT	50.0	45.0	57.8	58.0	215-243 bp	1.0	81.4
ndhD-psaC_p2	GAACCCGCGGATATTGGTAA	GGCATGAAACAACTCGAAGC	50.0	50.0	57.8	58.0	221-249 bp	1.0	81.2
ndhD-psaC_p3	GAACCCGCGGATATTGGTAA	AGAACGGAAGACTGTGTTGG	50.0	50.0	57.8	57.8	300-328 bp	1.0	78.7
ndhD-psaC_p4	GAACCCGCGGATATTGGTAA	CCAAGAACGGAAGACTGTGT	50.0	50.0	57.8	57.8	303-331 bp	1.0	78.7
ndhD-psaC_p5	GAACCCGCGGATATTGGTAA	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.8	57.7	276-304 bp	1.0	78.2