Hotspot

ndhC-trnV-UAC

LSC · 50254-51533 · 1280 bp

Back to Xylosma

Pi 0.112

MarkerSeek score 90.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1280
variable_sites: 137
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGACCGGAAATTAGAAATGCCA	AGAAGGTCTACGGTTCGAGT	40.9	50.0	58.0	57.8	1402-1463 bp	1.0	73.2
ndhC-trnV-UAC_p2	AGACCGGAAATTAGAAATGCCA	GAAGGTCTACGGTTCGAGTC	40.9	55.0	58.0	57.5	1401-1462 bp	1.0	70.4
ndhC-trnV-UAC_p3	GACCGGAAATTAGAAATGCCA	AGAAGGTCTACGGTTCGAGT	42.9	50.0	56.6	57.8	1401-1462 bp	1.0	70.1
ndhC-trnV-UAC_p4	AGACCGGAAATTAGAAATGCCA	GTTCGAGTCCGTATAGCCCT	40.9	55.0	58.0	58.7	1390-1451 bp	1.0	69.4
ndhC-trnV-UAC_p5	AGACCGGAAATTAGAAATGCCA	AAGGTCTACGGTTCGAGTCC	40.9	55.0	58.0	58.8	1400-1461 bp	1.0	68.2