Hotspot

trnE-UUC-trnT-GGU

LSC · 30930-31159 · 230 bp

Back to Xylosma

Pi 0.0856

MarkerSeek score 77.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 19
pi: 0.0856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	291-309 bp	1.0	84.4
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	288-306 bp	1.0	84.3
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	312-330 bp	1.0	84.1
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	309-327 bp	1.0	84.0
trnE-UUC-trnT-GGU_p5	TCGTAATACCCTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	57.6	58.1	348-366 bp	1.0	82.4