Hotspot

trnY-GUA-trnE-UUC

LSC · 30799-30856 · 58 bp

Back to Xylosma

Pi 0.0345

MarkerSeek score 67.80

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 2
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGATTTGAACCAGCGTAGG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.9	58.3	177-182 bp	1.0	77.9
trnY-GUA-trnE-UUC_p2	ATTTGAACCAGCGTAGGCAT	CTCTCTTTCAAGGAGGCAGC	45.0	55.0	57.9	58.3	174-179 bp	1.0	77.8
trnY-GUA-trnE-UUC_p3	TGGATTTGAACCAGCGTAGG	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.9	57.7	198-203 bp	1.0	77.7
trnY-GUA-trnE-UUC_p4	ATTTGAACCAGCGTAGGCAT	TCGTCTAGTGGTTCAGGACA	45.0	50.0	57.9	57.7	195-200 bp	1.0	77.5
trnY-GUA-trnE-UUC_p5	ATCAGTGAATTATCGGGCCG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	200-205 bp	1.0	77.3