Hotspot

petN-psbM

LSC · 27589-28879 · 1291 bp

Back to Xylosma

Pi 0.0106

MarkerSeek score 67.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1291
variable_sites: 13
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1453-1473 bp	1.0	71.6
petN-psbM_p2	CTTGGGCTGCTTTGATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1441-1461 bp	1.0	71.6
petN-psbM_p3	TTGGGCTGCTTTGATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1440-1460 bp	1.0	71.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1392-1412 bp	1.0	70.2
petN-psbM_p5	GGGGCCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1475-1495 bp	1.0	69.7