Hotspot

atpH-atpI

LSC · 12565-13584 · 1020 bp

Back to Xylosma

Pi 0.0129

MarkerSeek score 67.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 13
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GTGAATCCATGGAGGGACAC	45.0	55.0	58.0	58.0	1077-1078 bp	1.0	81.1
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	GTGAATCCATGGAGGGACAC	45.0	55.0	57.9	58.0	1069-1070 bp	1.0	80.9
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1106-1107 bp	1.0	80.4
atpH-atpI_p4	GTCCAATAGAAGCAAGCCCA	GTGAATCCATGGAGGGACAC	50.0	55.0	57.9	58.0	1115-1116 bp	1.0	80.3
atpH-atpI_p5	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1098-1099 bp	1.0	80.2