Hotspot

trnS-GCU-trnR-UCU

LSC · 7149-8767 · 1619 bp

Back to Xylosma

Pi 0.0188
MarkerSeek score 63.00
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1619
variable_sites
30
pi
0.0188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 ATTAGCAATCCGGCGCTTTA AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.0 56.9 1695-1808 bp 1.0 65.6
trnS-GCU-trnR-UCU_p2 ATCAACGGAACCGGAAAGAG AGAAGACCTCTGTCCTATCCA 50.0 47.6 57.9 56.9 1754-1867 bp 1.0 65.1
trnS-GCU-trnR-UCU_p3 ATTAGCAATCCGGCGCTTTA AGGTTTAGAAGACCTCTGTCCT 45.0 45.5 58.0 57.9 1701-1814 bp 1.0 64.9
trnS-GCU-trnR-UCU_p4 ATCAACGGAACCGGAAAGAG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 57.9 57.9 1760-1873 bp 1.0 64.4
trnS-GCU-trnR-UCU_p5 CATCAACGGAACCGGAAAGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1755-1868 bp 1.0 64.3