Hotspot

trnH-GUG-psbA

LSC · 76-327 · 252 bp

Back to Xylosma

Pi 0.0826

MarkerSeek score 88.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 20
pi: 0.0826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 88.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AACCGTGCAAACCTTGGTAT	55.0	45.0	58.1	58.0	373-400 bp	1.0	85.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AACCGTGCAAACCTTGGTAT	50.0	45.0	57.9	58.0	387-414 bp	1.0	84.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AACCGTGCAAACCTTGGTAT	50.0	45.0	57.9	58.0	386-413 bp	1.0	84.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AACCGTGCAAACCTTGGTAT	50.0	45.0	57.9	58.0	388-415 bp	1.0	84.8
trnH-GUG-psbA_p5	GCGAATGACGGGAATTGAAC	AACCGTGCAAACCTTGGTAT	50.0	45.0	57.8	58.0	425-540 bp	1.0	83.5